Posttranscriptional regulation is important in the controlling of flowering time in Arabidopsis. FCA gene product like an RNA binding protein and the other processes of FCA transcript shows that posttranscriptional regulation is important. The RNA binding proteins function in processing like, splicing, nuclear export, RNA stability, translation and polyadenylation.
WW protein – protein interaction displays that FCA also interacts with other proteins, like with proline-rich ligand. fca-1 mutations shows that this protein is important for FCA function. WW containing proteins functions to bring proteins together intracellular in regulatory pathways. Knowing which proteins bind to the FCA WW domain will help to find out what the function of the domain is in the FCA protein.
Experimental data shows that transcript y shows that it is the only transcript that encodes a functional FCA protein. FCA transcript is spliced and four mRNAs were detected, suggesting that FCA is a component of the posttranscriptional regulatory cascade. When the transcription of the FCA gene was increased, very high amount of transcript β levels, small increases in transcript y and δ levels resulted in an acceleration in flowering time. The flowering time of the 35S-FCA was ealier than the wild type. This may have been due to the fact there was an increase in levels of transcript y.
In conclusion FCA functions in the posttranscriptional regulation of transcripts that were involved in flowering process. The plants that had the FCA gene, which was fused to the 35S promoter flowered earlier, also the ratio and the concentration of the different FCA transcripts were changed. FCA is a component of the posttranscriptional cascade that is involved in th econtrol of flowering time in Arabidopsis.
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